Mouse Cytochrome P450 27B1 ELISA Kit from Innovative Research is intended for the quantitative determination of Mouse Cytochrome P450 27B1 in biofluid samples, such as tissue homogenates, cell lysates and other biological fluids. This is a sandwich ELISA in strip well format with reagents for up to 96 tests . CYP27B1 present in samples reacts with an anti-Mouse CYP27B1 capture antibody coated and dried on a microtiter plate. After appropriate washing steps, an anti-CYP27B1 detection antibody conjugated to biotin binds to the captured analyte. Excess antibody is washed away and bound antibody is then reacted with avidin conjugated to horseradish peroxidase. TMB substrate is used for color development at 450nm. Color development is directly proportional to the concentration of active CYP27B1 in the samples. A standard calibration curve is prepared along with the samples to be measured using dilutions of Mouse CYP27B1. Detection Target: Mouse Cytochrome P450 27B1 Reactivity: Mouse Range: 0.625-40ng/mL Sensitivity: 0.214ng/mL Storage Conditions: Store all components at 4┬░C This Mouse CYP27B1 activity assay is for the quantitative determination of Cytochrome P450 27B1 in samples including tissue homogenates, cell lysates and other biological fluids. For research use only. Strip well format. Reagents for up to 96 tests. For research use only. At Innovative Research, we provide reliable, consistent products that deliver reliable, consistent results.